Journal: Journal of Clinical Microbiology

Article Title: Genomics Reveals the Worldwide Distribution of Multidrug-Resistant Serotype 6E Pneumococci

doi: 10.1128/JCM.00744-15

Figure Lengend Snippet: Reference PMEN ^a genomes and cps locus sequences used in this study

Article Snippet: PMEN17 , Maryland 6B -17 , ST384 , ATCC BAA342; ENA , 1997 , Invasive disease isolate, United States , 10608770.

Techniques: Isolation

Journal: Scientific reports

Article Title: Development of a diagnostic assay by three-tube multiplex real-time PCR for simultaneous detection of nine microorganisms causing acute respiratory infections.

doi: 10.1038/s41598-022-15543-6

Figure Lengend Snippet: Figure 4. Diagnostic performance evaluation of the multiplex real-time RT-PCR. A, schematic representation of flow chart of the multiplex real-time PCR assay for detection of clinical specimens. After the patient’s nasopharyngeal/throat swab is taken, the nucleic acid extracted from the sample is amplified simultaneously in three tubes by the multiplex PCR assay. If the fluorescence channel of RSV in tube A shows an amplification curve and GAPDH is detected in all three tubes, a valid sample collection can be diagnostic for RSV infection in the sample. B, the number of each pathogen detected by three-tube nine-plex real-time PCR. The total number of pathogens detected in each type of infection were as follows: Single, 7; Dual, 7; Triple, 7; Quadruple, 4. LP: Legionella pneumophila, HI: Haemophilus influenzae, MP: Mycoplasma pneumoniae, CP: Chlamydia pneumoniae, SP: Streptococcus pneumoniae.

Article Snippet: Number Species Subtypes Strain ID Supplier Description 1 Influenza A virus H1N1 ATCC VR95 American Type Culture Collection 2 H3N2 GDV124 The State Key Laboratory of Virology, Wuhan University, CHINA3 H1N1 2009 GDV108 4 H5N1 The National Institute for Food and Drug Control, CHINA Supplied as virus cultures5 H7N9 6 Influenza B virus IVB Yamataga GDV105 The State Key Laboratory of Virology, Wuhan University, CHINA7 IVB Victoria GDV104 8 Respiratory syncytial virus RSV A ATCC VR1540 American Type Culture Collection 9 RSV B ATCC VR 955 10 Adenovirus Human adenovirus 1 ATCC VR-1 American Type Culture Collection 11 Human adenovirus 2 ATCC VR-846 12 Human adenovirus 3 ATCC VR-3 13 Human adenovirus 4 ATCC VR-1572 14 Human adenovirus 5 ATCC VR-5 15 Human adenovirus 46 ATCC VR-1308 16 Human adenovirus 48 ATCC VR-1406 17 Human mastadenovirus 7 ATCC VR-7 18 Human adenovirus 55 N/A The State Key Laboratory of Virology, Wuhan University, CHINA Supplied as clinical isolates 19 Chlamydia pneumoniae ATCC VR2282 American Type Culture Collection 20 Mycoplasma pneumoniae Mycoplasma pneumoniae Somerson et al ATCC 15531 21 Streptococcus pneumoniae Streptococcus pneumoniae Serotype 19F ATCC 49619 22 Streptococcus pneumoniae (Klein) Chester ATCC 700669 23 Legionella pneumoniae Legionella pneumophila subsp. fraseri Brenner et al ATCC 33156 24 Legionella pneumophila subsp. pneumophila Brenner et al ATCC 43290 25 Haemophilus influenzae Haemophilus influenzae (Lehmann and Neumann) Winslow et al ATCC 10211 12 Vol:. (1234567890) Scientific Reports | (2022) 12:13306 | https://doi.org/10.1038/s41598-022-15543-6 real-time RT-PCR assay followed the pattern of selecting conserved gene sequence regions.

Techniques: Diagnostic Assay, Multiplex Assay, Quantitative RT-PCR, Real-time Polymerase Chain Reaction, Amplification, Fluorescence, Infection

Journal:

Article Title: Comparison and Evaluation of Real-Time PCR, Real-Time Nucleic Acid Sequence-Based Amplification, Conventional PCR, and Serology for Diagnosis of Mycoplasma pneumoniae

doi: 10.1128/JCM.41.9.4366-4371.2003

Figure Lengend Snippet: Bacterial species and strains

Article Snippet: Streptococcus pneumoniae , ATCC 49150 , ATCC.

Techniques:

Journal:

Article Title: Comparison and Evaluation of Real-Time PCR, Real-Time Nucleic Acid Sequence-Based Amplification, Conventional PCR, and Serology for Diagnosis of Mycoplasma pneumoniae

doi: 10.1128/JCM.41.9.4366-4371.2003

Figure Lengend Snippet: Ct values for M. pneumoniae dilution series after duplex real-time PCR amplification with and without the PhHV spike

Article Snippet: Streptococcus pneumoniae , ATCC 49150 , ATCC.

Techniques: Real-time Polymerase Chain Reaction, Amplification

Journal:

Article Title: Detection of Low Levels of Listeria monocytogenes Cells by Using a Fiber-Optic Immunosensor

doi: 10.1128/AEM.70.10.6138-6146.2004

Figure Lengend Snippet: Specificity of the immunosensor for L. monocytogenes when compared with Listeria spp. and other microorganisms a

Article Snippet: When the sensor was evaluated for its reaction with other Listeria spp. at approximate concentrations of 4 × 10 9 CFU/ml each, L. monocytogenes showed a significantly ( P < 0.05) stronger signal (1,728 pA) than L. ivanovii (346 pA), L. grayi (204 pA), L. seeligeri (381 pA), L. welshimeri (533 pA), or L. innocua (720 pA) (Table ). table ft1 table-wrap mode="anchored" t5 TABLE 2. caption a7 Species or control Density (CFU/ml) Mean signal strength (pA) ± SD b L. monocytogenes V7 4.0 × 10 9 1,728 ± 325 A L. ivanovii ATCC 19119 4.0 × 10 9 346 ± 85 D L. grayi ATCC 19120 4.0 × 10 9 203 ± 9 D L. seeligeri SE 31 4.0 × 10 9 381 ± 186 BCD L. welshimeri ATCC 35897 4.0 × 10 9 533 ± 57 BC L. innocua F4248 4.0 × 10 9 720 ± 11 B Control (PBS) 0 114 ± 87 D L. monocytogenes V7 ∼10 9 2,179 ± 366 A S. enterica serovar Typhimurium ∼10 9 553 ± 77 B E. coli ATCC 52739 ∼10 9 374 ± 39 BC Enterococcus faecalis ATCC 344 ∼10 9 110 ± 41 CD Lactobacillus plantarum NCDO 955 ∼10 9 310 ± 82 BCD Serratia marcescens ATCC 14756 ∼10 9 147 ± 82 CD H. alvei ∼10 9 73 ± 51 D Enterobacter aerogenes ∼10 9 141 ± 28 CD P. aeruginosa ATCC 10145 ∼10 9 327 ± 44 BCD Corynebacterium glutamicum ATCC 31834 ∼10 9 281 ± 58 BCD Carnobacterium gallinarum ATCC 49517 ∼10 9 342 ± 78 BCD Control (PBS) 0 347 ± 12 BCD Open in a separate window a Bacterial cultures were precultured at 37°C and with 0.5% NaCl. b Mean signal strengths were based on the average of results from three independent measurements (fibers) from a representative experiment.

Techniques: Control

Journal:

Article Title: Detection of Low Levels of Listeria monocytogenes Cells by Using a Fiber-Optic Immunosensor

doi: 10.1128/AEM.70.10.6138-6146.2004

Figure Lengend Snippet: Fiber-optic signal acquisition for L. monocytogenes in mixture in PBS, pH 7.2 a

Article Snippet: When the sensor was evaluated for its reaction with other Listeria spp. at approximate concentrations of 4 × 10 9 CFU/ml each, L. monocytogenes showed a significantly ( P < 0.05) stronger signal (1,728 pA) than L. ivanovii (346 pA), L. grayi (204 pA), L. seeligeri (381 pA), L. welshimeri (533 pA), or L. innocua (720 pA) (Table ). table ft1 table-wrap mode="anchored" t5 TABLE 2. caption a7 Species or control Density (CFU/ml) Mean signal strength (pA) ± SD b L. monocytogenes V7 4.0 × 10 9 1,728 ± 325 A L. ivanovii ATCC 19119 4.0 × 10 9 346 ± 85 D L. grayi ATCC 19120 4.0 × 10 9 203 ± 9 D L. seeligeri SE 31 4.0 × 10 9 381 ± 186 BCD L. welshimeri ATCC 35897 4.0 × 10 9 533 ± 57 BC L. innocua F4248 4.0 × 10 9 720 ± 11 B Control (PBS) 0 114 ± 87 D L. monocytogenes V7 ∼10 9 2,179 ± 366 A S. enterica serovar Typhimurium ∼10 9 553 ± 77 B E. coli ATCC 52739 ∼10 9 374 ± 39 BC Enterococcus faecalis ATCC 344 ∼10 9 110 ± 41 CD Lactobacillus plantarum NCDO 955 ∼10 9 310 ± 82 BCD Serratia marcescens ATCC 14756 ∼10 9 147 ± 82 CD H. alvei ∼10 9 73 ± 51 D Enterobacter aerogenes ∼10 9 141 ± 28 CD P. aeruginosa ATCC 10145 ∼10 9 327 ± 44 BCD Corynebacterium glutamicum ATCC 31834 ∼10 9 281 ± 58 BCD Carnobacterium gallinarum ATCC 49517 ∼10 9 342 ± 78 BCD Control (PBS) 0 347 ± 12 BCD Open in a separate window a Bacterial cultures were precultured at 37°C and with 0.5% NaCl. b Mean signal strengths were based on the average of results from three independent measurements (fibers) from a representative experiment.

Techniques: Control